The EasiLV system (Goujon et al, Nature 2013)
This is a lentiviral vector containing an antisense and inducible expression cassette for your favorite gene of interest. An E2-crimson fluorescent reporter allows the easy detection of the transduced cells by FACS or microscopy. This system prevents transcription of the cDNA of interest in the EasiLV producer cells and allows expression, whenever needed, in transduced cells. This system if of particular interest when working with a gene potentially cytotoxic or with antiviral properties. This vector is available upon request ([email protected]).
This is a lentiviral vector containing an antisense and inducible expression cassette for your favorite gene of interest. An E2-crimson fluorescent reporter allows the easy detection of the transduced cells by FACS or microscopy. This system prevents transcription of the cDNA of interest in the EasiLV producer cells and allows expression, whenever needed, in transduced cells. This system if of particular interest when working with a gene potentially cytotoxic or with antiviral properties. This vector is available upon request ([email protected]).